{ "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "FrameKey-0-0-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9a256dd8-9ec2-43e4-ad24-c64a589ebc12", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6602835, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:45:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9c6a118c-e05e-4c84-ba6e-5a09bebae15b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6603307, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:45:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bec75806-9f12-4960-8046-03a2bff5d387", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6603776, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:45:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "68530527-9b4e-4b6e-aca1-e396e2043312", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6604264, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:45:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "579260f6-2e1b-4fec-98e8-5fc56d9efd8f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6604734, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:45:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8c03927d-f009-47f2-9a1d-e0f6ba9615b9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6605209, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:45:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "144e8e9f-4f22-496d-ac52-0feb5821fc0e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6605679, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:45:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cb1698cf-e55e-428e-8503-b5930f224701", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6606149, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:45:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "83340cac-8a4e-417e-b6ef-ab5713cee513", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6606618, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:45:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e178e4e7-82f1-429f-b78c-7fb101abe851", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6607097, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:45:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "66b8a209-1326-4794-b739-d8f8a69ef074", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6607578, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:45:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3ea98a22-c468-4801-b89f-2e19d3a590d4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6608048, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cbd62113-a6d4-4847-8f7b-ae796c4f180e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6608519, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3fac3205-3304-4ba9-925c-f08dec4a9de2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6608988, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "63f9e427-9466-4320-b02f-2ad0b7f0efd7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6609459, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e5878a68-36b0-43e3-b99e-ef8c3a1dd20f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6609930, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d8bba32c-3161-48b5-b9a6-e3081d33b103", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6610400, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f5af8972-3edf-452d-ae9c-22d72ae3b0ed", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6610869, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "024080fe-690a-4025-8b97-100a4fdacbae", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6611339, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8e5c4775-abf5-443f-bf7d-b72bb13c3c92", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6611810, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6d7db124-eb5f-4c77-a021-e4b685579270", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6612282, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b774f4b5-0f14-4f3e-b637-dbf25bdf415d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6612752, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "35a0f90e-17eb-4c46-a311-a6f11d0a035a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6613223, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4c938902-5ced-4f5c-b8bc-281476369917", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6613693, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "843274ee-8554-4ce3-9aed-7c71b59d8bbe", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6614164, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "05d80862-85d7-499e-98bc-3908206f1b06", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6614635, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ca43d7f2-a425-42c9-ae5f-2fd51030ff24", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6615104, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d24c1a03-c2c0-459f-910c-9a8f4ec785e0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6615573, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "49fd792e-9020-41ed-b594-2e63ceb6fbc7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6616045, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c84eab9b-dde4-4dd0-a0e1-2d04499afae9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6616527, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8955bf09-2fa6-4d29-bd86-84bc6f65f94c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6616997, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "81f8f979-1d35-4642-9f60-9298b27ed8b1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6617466, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dae282b9-d128-43fd-91c0-c3910a58676b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6617937, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e216408c-029f-4181-b113-6fcb5b710b24", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6618424, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d1544c7d-0f93-4a97-9281-b38200fd08b7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6618894, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5bc1509d-ab24-4262-a047-919d10ad7ddc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6619363, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "83d6234c-9c61-4b4b-a424-55363e536715", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6619834, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a21d217b-6c31-4366-b0d1-92525c91ee0d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6620309, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c24c7ab1-d792-4e1e-9e13-d4d9bfdd0f92", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6620781, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8a44a9f0-cd3c-4481-abcd-3e0c981decb7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6621252, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "70ed6093-876e-40ae-b02f-d5dfc5cba5d4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6621722, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8d6a54c6-7092-4ae5-9b89-e8a347a440de", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6622745, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a0575c8a-7d8d-4356-a2e4-43c66a1a1baf", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6622996, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7801faa2-fd5c-45d9-8c8c-7f3bb1421203", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6623247, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f815b7c2-9a7b-4cff-80bf-ad97a26e687a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6623499, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "62bbd1f5-e04b-424f-a07e-31084adfe5f1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6623750, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1ae2edb5-0d6b-4a83-97b5-19f548824e55", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6624001, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "21eae504-6d38-45b8-86fb-11d22b69f87c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6624250, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fe5610b9-99ca-47e6-9e33-0f6925b3fbab", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6624518, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4bd49a49-0383-4320-b42b-31b17cb840b2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6624770, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "53f7ed21-c6a4-45a3-8242-1e32ff1342ea", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6625021, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "505643fc-a97d-44e7-a152-01e0ab4c311e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6625272, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6cf878ba-653d-4e70-8717-d8eb98822091", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6625524, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e006c456-f696-420e-886a-24307671e614", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6625774, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a3e9ca8a-ec61-4863-8adb-0069025cc6ee", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6626031, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b6ab8b2a-20bb-4aeb-bd34-1aff877c349e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6626282, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "566c5022-dd87-4b61-9291-3a0eb5ff62c4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6626532, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ce5a548f-7850-48e4-a40d-91d8605dc8c6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6626781, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "69e15327-9505-482f-bc54-0d56169591e8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6627031, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "511834d6-9234-41ac-a658-310be42876e4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6627283, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cff3a8a0-c5e5-46fb-862a-c87288f77c5a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6627534, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "93a93fc9-5454-4e7f-98ec-31eb06014a97", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6627785, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3d952d2e-d262-4725-9589-311c6ca99173", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6628035, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0ff4dcee-5662-46a9-b02a-40cc18fd8512", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6628287, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3e33855d-5cc1-48bb-9c4f-381c0ff3c41e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6628537, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0e882c9d-8e08-4264-8981-a66a2fc63895", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6628788, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "26c7dff5-e1f4-40a4-9844-27f5a9f1714d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6629038, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8c70cbe6-0f4d-4ab1-b057-336333ff84af", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6629289, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f75c73e9-5d01-4778-8641-f8b81bb960c2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6629539, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8bda9d99-5ed4-408d-bc89-d67faf383283", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6629790, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "32ddfa76-144e-4691-a5d5-2d52bd4a11b4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6630043, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b5eef360-fa62-4e17-bf0e-8c2f9df99a05", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6630294, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ad6ad1f5-ec00-45e9-8daf-58a03e743a78", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6630546, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4e372e24-cdb1-486e-80ef-15f4bb8e87e7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6630801, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b453704a-a174-4d62-b8e8-e1d5785eb7dd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6631052, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "77f26d02-16aa-4563-89fa-7521fb3c6473", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6631304, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2ff377a1-7d68-45cc-8f2a-22d660a957c0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6631555, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "147fe1b2-86f8-4797-be0a-70302c43abc6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6631808, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "edcc6c6e-0d16-4818-9f71-9b8663e2c9d8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6632060, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1588051d-96f9-44e2-a021-6f6cd6229008", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6632311, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "510d7843-0b07-4338-9bfa-597e3752e07e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6632562, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f6e7b334-ad81-48f7-b962-6b8df8ae9ef4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6632813, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "aa48cf6e-4357-414c-a5b5-6b045925fa48", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6633961, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2d8699fb-306b-472f-b1ed-e862abda380c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6634413, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a7b2bc55-051d-4bb9-bac1-9d460f1d7550", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6634865, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "923d4986-ad08-4164-bf7b-7ea5d8dd7f82", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6635316, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f05fb2ad-c1e6-4a08-9626-6530b7b90ca7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6635765, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "775f7204-bde0-4f03-a0f1-db90ac5c3772", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6636216, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "39c17e0f-f206-4779-8505-5e4c8478d2df", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6636668, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "43be56d5-d5e9-4b9e-b2d4-f7e0487ba0b3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6637118, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d9bbecf9-0dc2-4464-bd0b-f16c75a9c2f4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6637569, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "73128165-ddc3-42c0-be81-6c5c83bef848", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6638019, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f5bba9c9-025b-47b9-b401-b63613c6f8fe", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6638471, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "594adb50-03db-433c-94c8-84622b192d8a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6638923, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "08caf16e-63e8-4c22-8997-69a39995ca5a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6639373, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "16eede82-695c-4088-aef6-8c5fda181cce", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6639824, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a764285a-a301-4cb1-9168-072f0e0960cb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6640276, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7c7bff9a-b503-41b3-bf9f-b4b7436ee290", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6640727, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "60a11e15-3e01-4603-bf8a-3158085e12dd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6641186, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d13e1ca1-4836-477c-8868-12d2a4d72e87", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6641636, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "692d339c-0e57-4228-840d-0b923ce97b8b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6642088, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1374e14e-6686-4c99-8715-dd4727395122", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6642538, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "19f84baf-be55-4650-8b4f-f299528a09df", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6642988, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a93dd783-c2ae-4493-95fb-d9eb1c1a7638", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6643440, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a5cf11a2-8dba-4398-abae-53c91f14d903", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6643891, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a0138514-de44-42c7-bb4b-c1eebcdcbec7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6644342, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ddd45345-7293-4705-be02-0108aa2e95e6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6644792, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2314a1b9-44dd-42ab-b69b-d10126b40544", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6645242, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "21245067-0a79-4f5c-9a77-dc4cc310a1a5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6645701, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c68429c0-a0d2-4ab3-9f23-056a67689018", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6646150, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "de4be540-f05f-4cce-bedd-e6e80218b310", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6646598, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "562616e7-7869-4c9b-bed8-98daa82aa09f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6647058, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "72a5b711-5456-4043-8166-3e0254f1a28d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6647508, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "717604f3-372c-40b8-b55e-e7e62f030429", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6647960, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1679f0db-3e6b-4582-8e0d-416be5cf5d01", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6648416, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "730fb462-3b95-4e84-bec3-4e2ad5bc6442", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6648866, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7de0b890-911a-4eba-b317-42ce83376be7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6649315, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d75d80cf-6115-4aa4-bebf-962e5f1730c5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6649765, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d0a22d77-7e98-4a07-81a4-3a872a454d6d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6650216, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ee41a415-6087-4992-8754-0c4e8e8ca9fa", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6650665, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eb99215f-452f-46b7-9fad-9caa49931374", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6651114, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6c590f95-6d9e-4b41-b777-0a3aa4f9bed3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6651565, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b5cbc7d1-242c-4255-9946-05877cccdf87", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6652015, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4b452454-a578-45c1-8b49-cea2f3af656c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6652891, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9be20d2c-94c2-4edd-92b6-87dc5a8e80b8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6653480, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7200a748-00b5-49f0-8e06-144241b4d93a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6654070, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c1636f8d-0589-486a-ba9f-c1beec693fdb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6654660, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3a823d3d-ce40-4b63-a315-6c5331fa81b3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6655249, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8cf38668-b2f0-46bc-afa0-9fe60f390aa4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6655838, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9cba4bed-6480-4438-9e4d-c4e915f6d942", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6656428, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cb06799f-7d74-4eaf-910c-8fffd30ffd70", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6657017, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e1333413-c7e6-4314-9560-1ee8e523c9e4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6657606, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a1bc4067-a69d-41b8-aa12-e58b056a297b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6658196, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8ebc0478-ce07-4ede-a7c7-403612e7739c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6658787, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f46e8e40-2e82-418d-8067-66dd8a1a25ad", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6659375, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "812b10b3-a335-42cd-84d0-7d434a75cd31", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6659972, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dbe8d703-bc2f-4f51-834e-ee1b26ea51f7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6660561, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1a60978d-ea1a-48c1-a777-fd26a559b36b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6661155, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "216e73c2-a38d-47be-926d-6aacdacb84db", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6661746, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f8c4b625-af87-4f25-9905-41c25ba32031", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6662336, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e95ce60d-1e83-4f1e-97ae-18b9c77bd82d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6662926, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ca68258e-fcd1-403a-b281-65f161c30bc7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6663517, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4004ea39-e664-4a79-80e0-9bf16edd1394", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6664107, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f2ef483e-8952-4633-8450-7b0d48f7cb49", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6664696, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "054762b6-fdbd-4c5f-b763-9ce90928ebc9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6665286, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e1bff664-cd0f-4639-a7a0-57afa6ba11f0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6665886, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d3c80989-2243-4122-a6a1-0823c34d551b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6666474, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "82d6cebb-2178-4c2d-83d3-fd77914f4c06", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6667064, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3d8eb37a-409d-4186-98e5-4ec8a23cd68c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6667655, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:46:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "41767da7-ce2e-40ae-87a4-4d20086f1c85", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6668245, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4d5bd45d-3dc9-42f6-a6aa-d33d33d5904b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6668833, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f7f442d5-6f8d-4ca5-b558-d6f02f9a1c6c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6669422, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "181be484-1810-4f04-84ae-d07d9929952d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6670012, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8dd7e002-3caf-44f5-b48d-d29c0c3a3c56", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6670602, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1fbc1d9b-0744-4a18-9d6e-c8afe35f3c5a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6671202, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "825514d5-774d-4a80-ba30-325ecb29a25f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6671790, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b943b466-ae63-47be-82e5-fdbac03c7319", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6672382, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5b8ce640-861d-40b9-bd68-fb1ea206a3c5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6672973, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bcb068ac-153b-49e1-897a-4480b35da2ab", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6673564, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "33556ace-e6f4-498e-8ebc-d0f5da4b1d7a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6674155, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e5025064-3b73-4377-868b-bf6ff30b7ef3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6674744, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "93b5f85e-540a-4c66-8cf4-5d5dbc9b6e20", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6675340, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0c295794-02d3-454a-a707-069ffe001a01", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6675931, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ff0965f7-c87d-47e0-8980-c35a9ad0ba6d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6676522, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8a61a165-6623-4788-9601-9178667d77c3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6677641, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e43ced70-11ba-40c2-826d-1ec27f116e93", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6678089, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5e095d97-c145-46b1-9a9c-e2f2035ff6cf", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6678520, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a3e709f9-3c31-4b0f-a18b-6e066a761f9e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6678953, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c47050ec-ea3d-45a7-b495-8a53f95df8de", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6679384, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fd5bfbcd-7a11-4fec-8998-d941108f9528", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6679831, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ce3525de-c00f-4bb0-b761-3a4b0af053f0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6680281, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8ac81c31-dbe3-47c0-b11a-04d58d21dc41", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6680758, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9cfc41f7-427a-4ecc-9b11-44221a80674d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6681192, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f7de66d4-8075-4a84-9e0f-a6054f1d5856", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6681655, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eb691c1c-08eb-4367-b374-43d5f46414c4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6682104, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "893e36dd-57f7-4ce0-bfc1-2cd855ebe253", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6682568, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1b5c7550-0b93-49d6-a461-1e7396efe03a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6682999, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "09f265b9-f11b-44d3-b3fb-86a7a2a9b288", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6683432, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cb778362-4f49-43ae-ab3a-98f80514012f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6683879, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "568d469f-d6fc-4d1e-a6ae-6c0186179fea", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6684375, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "01635c86-770a-4dc7-9b3d-9a9361ab7dda", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6684840, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6862beb9-8023-4c4a-851a-0d7023be7c52", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6685304, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "30c816f9-104c-49a4-9ea9-8c5b4073a0df", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6685735, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1527d69f-3606-429a-a6d9-fdb85e72cab3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6686199, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3187a613-2b89-4610-864a-aad420bb471f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6686647, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b81ecb1d-c69a-4d85-b1bc-afc34b9e4214", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6687079, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "503f58e9-20e6-407a-9cc8-ea1f8fc8de5e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6687530, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9807ff76-67a3-43e9-bc81-355e3ca62bf8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6687990, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5dcf39e0-d843-4265-be4c-65ecba9c9a77", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6688437, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "06ed65f9-5ac8-4cac-9f0f-682638ab9642", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6688886, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1cbb0e2c-d49d-4005-9ffe-c12d44132ff5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6689334, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "35aa9fcf-2141-4067-921f-16b7ed24fc9f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6689783, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ab9b2f11-1f76-422b-9b6c-0acdabba3fc5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6690231, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f9b44e63-842b-4c6c-ae55-5d678ee58926", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6690695, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d2dddb24-6ff0-4d08-97be-3cd0412039c0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6691126, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b4a1ef51-8169-426c-a3db-a73bf5ae6b79", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6691590, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1f7f1317-023e-4bca-aea7-54681bba80aa", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6692053, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "aa69be09-61c6-4e50-8ab3-dc810bf59985", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6692510, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1e87b475-86f5-425f-9e36-5444c86c4714", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6692981, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "166d337b-4c8e-4adb-9094-4010c18f3e11", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6693415, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bdd57797-5523-4126-9e32-2f1ca8eaae18", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6693861, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d39dda7a-5b09-4ff3-8854-56fba05d550e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6694309, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c6fb58b1-d5d6-4ae4-ac30-191317111513", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6694774, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d29eddcf-621a-41d5-8746-e852a6ef9dd0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6695238, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 20, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8300", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c7b7edfb-5e4a-47e4-9ec1-c9e9c8229a12", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 70, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4745.0560000000005 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4745.0560000000005 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4745.0560000000005 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4745.0560000000005 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4745.0560000000005 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4466.528 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4466.528 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4466.528 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4466.528 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4466.528 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -4188 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -4188 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -4188 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -4188 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -4188 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3909.4720000000007 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3909.4720000000007 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3909.4720000000007 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3909.4720000000007 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3909.4720000000007 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9659.944, -3630.9440000000004 ] }, "GridColumnIndex": 0, "Label": "2-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 9938.472, -3630.9440000000004 ] }, "GridColumnIndex": 1, "Label": "2-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10217, -3630.9440000000004 ] }, "GridColumnIndex": 2, "Label": "2-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10495.528, -3630.9440000000004 ] }, "GridColumnIndex": 3, "Label": "2-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8397.99], "XYStage": [ 10774.056, -3630.9440000000004 ] }, "GridColumnIndex": 4, "Label": "2-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, -236.05600000000004 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, -236.05600000000004 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, -236.05600000000004 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, -236.05600000000004 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, -236.05600000000004 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 42.47199999999998 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 42.47199999999998 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 42.47199999999998 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 42.47199999999998 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 42.47199999999998 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 321 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 321 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 321 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 321 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 321 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 599.528 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 599.528 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 599.528 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 599.528 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 599.528 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4037.9440000000004, 878.056 ] }, "GridColumnIndex": 0, "Label": "3-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4316.472000000001, 878.056 ] }, "GridColumnIndex": 1, "Label": "3-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4595, 878.056 ] }, "GridColumnIndex": 2, "Label": "3-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 4873.528, 878.056 ] }, "GridColumnIndex": 3, "Label": "3-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8383.82], "XYStage": [ 5152.0560000000005, 878.056 ] }, "GridColumnIndex": 4, "Label": "3-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, -36.05600000000004 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, -36.05600000000004 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, -36.05600000000004 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, -36.05600000000004 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, -36.05600000000004 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 242.47199999999998 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 242.47199999999998 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 242.47199999999998 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 242.47199999999998 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 242.47199999999998 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 521 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 521 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 521 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 521 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 521 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 799.528 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 799.528 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 799.528 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 799.528 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 799.528 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9541.944, 1078.056 ] }, "GridColumnIndex": 0, "Label": "4-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 9820.472, 1078.056 ] }, "GridColumnIndex": 1, "Label": "4-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10099, 1078.056 ] }, "GridColumnIndex": 2, "Label": "4-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10377.528, 1078.056 ] }, "GridColumnIndex": 3, "Label": "4-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8390.81], "XYStage": [ 10656.056, 1078.056 ] }, "GridColumnIndex": 4, "Label": "4-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "7a9082fb-1b7e-4b79-8b02-758debaa321d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-08-15 16:45:54 -0400", "Date": "2019-08-15", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 70, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "genes_seq3_retakepos2-3-4_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -205, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 75, "Directory": "E:\\Dropbox\\ZadorConfocal1Data\\Yu-Chi\\20190814cadherinsandbarcode" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "100004000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6695686, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "4-Pos_000_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "20.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 9541.944, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8387.2700", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-08-15 16:47:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 1078.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "10.5000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" } }